ORIGINAL_ARTICLE
Bioactivity of some regular and Nano encapsulated essential oils against C. maculatus and C. chinensis
Callosobruchus maculatus (Cowpea weevil) and Callosobruchus chinensis (Bean weevil) are among the important pests of stored seeds. These insects are usually controlled by harmful insecticides. Essential oils are considered a safe substitute in controlling stored products pests. In this work, the Nano-encapsulation technique was used to enhance the effectiveness of three types of essential oils (Mentha piperita “Peppermint”, Coriandrum sativum “coriander”, and Laurus nobilis “sweet bay”). The effectiveness of both regular and Nano oils was evaluated for their insecticidal activities against the third-instar larvae of C. maculatus and C. chinensis at a concentration of 0.5%. Additionally, the ovipositional activity inhibitory effects of Nano granules were evaluated using by mixing Nano granules with cowpea at a rate of 0.5%. Furthermore, the persistent effect of Nano encapsulated oils was evaluated after 90 days in storage. The repellent effect of Nano granules was also evaluated using the arena choice test method. The highest mortality rates of both C. maculatus and C. chinensis after 7 days of 0.5% treatment were observed in M. piperita, followed by C. sativum and Laurus nobilis in both regular and Nano oils. A significant increase in the larvicidal effect in C. sativum Nano granules in comparison to its regular oil was noticed. The results indicated that both C. maculatus and C. chinensis life cycles were heavily affected by Nano granules treatments of the three tested Nano granules with M. piperita Nano granules having the highest impact. The lowest weight loss in stored cowpea was observed under M. piperita Nano granules treatment especially against C. maculatus (3%) compared to control treatment (50%). The highest repellent effect was observed under C. sativum treatment against C. chinensis (88%). These results are highly promising and show the potentials of these oils and their Nano derivatives in stored products integrated pest management.
https://life.dysona.org/article_113052_f1ac583f15b7da94eea231399ecb2995.pdf
2020-10-01
96
101
10.30493/dls.2020.239285
Cowpea weevil
Bean weevil
Mentha piperita
Laurus nobilis
Coriandrum sativum
Nano
Magda
Sabbour
sabbourm9@yahoo.com
1
Department of Pests and Plant Protection, National Research Center, Cairo, Egypt
LEAD_AUTHOR
Murdock LL, Shade RE. Eradication of cowpea weevil (Coleoptera: Bruchidae) in cowpeas by solar heating. Am. Entomol. 1991;37(4):228-31. DOI
1
Wilson K, Hill L. Factors affecting egg maturation in the bean weevil Callosobruchus maculatus. Physiol. Entomol. 1989;14(1):115-26. DOI
2
Isman MB. Plant essential oils for pest and disease management. Crop Prot. 2000;19(8-10):603-8. DOI
3
Kim SI, Yoon JS, Jung JW, Hong KB, Ahn YJ, Kwon HW. Toxicity and repellency of origanum essential oil and its components against Tribolium castaneum (Coleoptera: Tenebrionidae) adults. J Asia-Pac Entomol. 2010;13(4):369-73. DOI
4
Negahban M, Moharramipour S, Sefidkon F. Chemical composition and insecticidal activity of Artemisia scoparia essential oil against three coleopteran stored-product insects. J Asia-Pac Entomol. 2006;9(4):381-8. DOI
5
Moretti MD, Sanna-Passino G, Demontis S, Bazzoni E. Essential oil formulations useful as a new tool for insect pest control. AAPs PharmSciTech. 2002;3(2):64-74. DOI
6
Clancy KM, Foust RD, Huntsberger TG, Whitaker JG, Whitaker DM. Technique for using microencapsulated terpenes in lepidopteran artificial diets. J. Chem. Ecol. 1992;18(4):543-60. DOI
7
Passino GS, Bazzoni E, Moretti MD. Microencapsulated essential oils active against indianmeal moth. Boletin de Sanidad Vegetal Plagas. 2004;30:125-32.
8
Trenkel ME. Controlled-release and stabilized fertilizers in agriculture. Paris: International Fertilizer Industry Association; 1997.
9
Negahban M, Moharramipour S, Zandi M, Hashemi SA. Fumigant properties of nano-encapsulated essential oil from Artemisia sieberi on Tribolium castaneum. InProc 2012;9:15-9
10
El-Aziz A, El-Ghany A. Impact of Diatomaceous Earth Modifications for Controlling the Granary Weevil, Sitophilus granarius (Linnaeus)(Coleoptera: Curculionidae). J Agr Sci Tech-Iran. 2018;20(3):519-31.
11
Sabbour MM, Abd El-Aziz SE. Screening effects of three natural oils and their nano against Ephestia kuehniella (Lepidoptera-Pyralidae) in laboratory and store. Biosci. Res. 2017;14(2):408-16.
12
Lwande W, Hassanali A, Njoroge PW, Bentley MD, Delle Monache F, Jondiko JI. A new 6a-hydroxypterocarpan with insect antifeedant and antifungal properties from the roots of Tephrosia hildebrandtii Vatke. Int J Trop Insect Sci. 1985;6(4):537-41. DOI
13
Sokal RR. The principles and practice of statistics in biological research. Biometry. 1995:451-554.
14
Rajendran S, Sriranjini V. Plant products as fumigants for stored-product insect control. J. Stored Prod. Res. 2008;44(2):126-35. DOI
15
Huang J, Li Q, Sun D, Lu Y, Su Y, Yang X, Wang H, Wang Y, Shao W, He N, Hong J. Biosynthesis of silver and gold nanoparticles by novel sundried Cinnamomum camphora leaf. Nanotechnology. 2007;18(10):105104. DOI
16
Sabbour MM, Abd-El-Raheem MA. Repellent Effects of Jatropha curcas, canola and Jojoba Seed oil, against Callosobruchus maculates (F.) and Callosobruchus chinensis (L.). J. Appl. Sci. Res. 2013;9(8):4678-82.
17
Sabbour MM, Abd-El-Rahman AA, Ragei MA. Determinations of some extracted oils in controlling two stored product insect pests. Middle East J. Agric. Res. 2013;2(4):127-32.
18
Sabbour MM, Abd El-Aziz SE. Impact of certain nano oils against Ephestia kuehniella and Ephestia cutella (Lepidoptera-Pyralidae) under laboratory and store conditions. Bull Natl Res Cent. 2019;43(1):80. DOI
19
ORIGINAL_ARTICLE
Neuroprotective effects of α-Lipoic acid alone and in combination with ferulic acid in diabetic neuropathy induced rats
The present pre-clinical activity was undertaken to screen the two antioxidants, mainly α-lipoic acid and ferulic acid alone, and in combination in neuropathic pain induced by diabetes in rats. The activity was confirmed by assessing various behavioral as well as biochemical and histopathological studies. The study was performed on adult albino rats. The rats were divided into different groups, and each group contained six rats. Diabetic neuropathy in rats was induced by administering a freshly prepared single dose of streptozotocin (60 mg/kg, i.p). After the development of neuropathy, three groups were treated with α-lipoic acid (25 mg/kg/day, p.o), ferulic acid (10 mg/kg/day, p.o), or the standard drug Pregabalin (30 mg/kg/day i.p). The final group received a combination of antioxidants, (α-lipoic acid and ferulic acid) at 12 and 5 mg/kg, p.o, respectively, for two weeks. Neuropathic pain was assessed using mechanical allodynia, mechanical hyperalgesia, cold allodynia, and thermal allodynia. Biochemical parameters of blood glucose, nitric oxide, level of lipid peroxidase, reduced glutathione, and membrane-bound ATPase activities were also studied. Neuropathic pain-induced rats showed a significant alteration in behavioral and biochemical parameters. Treatment with α-lipoic acid in combination with Ferulic acid significantly restored the altered parameters towards normal as compared to single antioxidants, thus providing proper neuroprotection. This effect might be due to the strong free radical scavenging potential of α-lipoic acid and ferulic acid.
https://life.dysona.org/article_114609_e831c004dace07067f2c4db6511243b3.pdf
2020-10-01
102
112
10.30493/dls.2020.243982
neuropathic pain
α-lipoic acid
ferulic acid
oxidative stress
Streptozotocin
Sneha
Gupta
snehagupta1110@gmail.com
1
Department of Pharmacology, SNJBs SSDJ College of Pharmacy, Savitribai Phule Pune University, Pune, India
AUTHOR
Abdullah
Sherikar
abdullasherikar@rediffmail.com
2
Department of Pharmacology, SNJBs SSDJ College of Pharmacy, Savitribai Phule Pune University, Pune, India
AUTHOR
Aman
Upaganlawar
amanrxy@gmail.com
3
Department of Pharmacology, SNJBs SSDJ College of Pharmacy, Savitribai Phule Pune University, Pune, India
LEAD_AUTHOR
Chandrashekhar
Upasani
cdupasani@gmail.com
4
Department of Pharmacology, SNJBs SSDJ College of Pharmacy, Savitribai Phule Pune University, Pune, India
AUTHOR
Bhadada SK, Sahay RK, Jyotsna VP, Agrawal JK. Diabetic Neuropathy: Current Concepts. J IndClinMedi. 2001; 2(4):305-18.
1
Upaganlawar A. Supplementation of corosolic acid prevents the development of neuropathic pain in streptozotocin induced diabetic rats. J Pharmacol Clinic Sci.2016;1(1):11-8.
2
Horowitz SH. Textbook of diabetic neuropathy, edited by FA Gries, NE Cameron, PA Low, and D. Ziegler, Stuttgart, Thieme, 2003. Muscle & Nerve: Official Journal of the American Association of Electrodiagnostic Medicine. 2004;30(2):247-. DOI
3
Taliyan R, Sharma PL. Diabetic Neuropathic Pain: An Update and Novel Pharmacological Strategies for Relief of Pain. J. Med. Sci. 2010;10(4):93-109. DOI
4
Kolgazi M, Jahovic N, Yüksel M, Ercan F, Alican I. α‐Lipoic acid modulates gut inflammation induced by trinitrobenzene sulfonic acid in rats. J. Gastroenterol. Hepatol. 2007;22(11):1859-65. DOI
5
Biewenga GP, Haene GR, Bast A. The pharmacology of the antioxidant lipoic acid. Gen. Pharmacol. 1997;29:315-31. DOI
6
Bustamante J, Lodge JK, Marcocci L, Tritschler HJ, Packer L, Rihn BH. α-Lipoic acid in liver metabolism and disease. Free Rad. Biol. Med. 1998;24(6):1023-39. DOI
7
Packer L. α-Lipoic acid: a metabolic antioxidant which regulates NF-κB signal transduction and protects against oxidative injury. Drug Metab. Rev. 1998;30(2):245-75. DOI
8
Rochette L, Ghibu S, Richard C, Zeller M, Cottin Y, VergelyC.Direct and Indirect Antioxidant Properties of α-Lipoic Acid and Therapeutic Potential. Mol. Nutr. Food Res. 2013;57(1):114-25. DOI
9
Gajdosik A, Gajdosikova A, Stefek M, Navarova J, Hozova R. Streptozotocin-induced experimental diabetes in male Wistar rats. Gen. Physiol. Biophys. 1999;18:54-62.
10
Cory T. Pregabalin: latest safety evidence and clinical implications for the management of neuropathic pain. Ther Adv Drug Saf. 2014;5(1):38-56. DOI
11
Attal N, Cruccu G, Baron RA, Haanpää M, Hansson P, Jensen TS, Nurmikko T: EFNS guidelines on the pharmacological treatment of neuropathic pain: 2010 revision. Eur J Neurol. 2010;17(9):1113-e88. DOI
12
Rahaman H, Upaganlawar A, Upasani C. Protective effects of Ferulic acid alone and in combination with ascorbic acid on Aniline induced spleen toxicity. Anna of Pharmacol Pharmaceut. 2017. 2(1):1012-6.
13
Khairnar U, Upaganlawar A, Upasani C. Ameliorative effect of chronic supplementation of protocatechuic acid alone and in combination with ascorbic acid in aniline hydrochloride induced spleen toxicity in rats. Scientifica. 2016;2016. DOI
14
Upaganlawar A, Balaraman R. Cardioprotective effects of co-administration of Pomegranate extract and Vitamine E on electrocardiographic, biochemical and apoptotic changes in isoproterenol induced myocardial infarction in rats. Pharmacol; 2015;6(5):178-85. DOI
15
Upaganlawar A, Balaraman R. Combined effects of Vitamin E and lycopene on lipid profile and infarction size in isoproterenol induced cardiotoxicity in rats. Pharmacol. 2012; 3(7);215-20.
16
Raposo D, Morgado C, Pereira-Terra P, Tavaresa I. Nociceptive spinal cord neurons of laminae I–III exhibit oxidative stress damage during diabetic neuropathy which is prevented by early antioxidant treatment with epigallocatechin-gallate (EGCG). Brain Res. Bull. 2015;110: 68–75. DOI
17
Chaplan SR, Bach FW, Pogrel JW, Chung JM, Yaksh TL. Quantitative assessment of tactile allodynia in the rat paw. J. Neurosci. Methods. 1994;53(1):55-63. DOI
18
Blackburn-Munro G, Ibsen N, Erichsen HK. A comparison of the anti-nociceptive effects of voltage-activated Na+ channel blockers in the formalin test. Eur. J. Pharmacol. 2002;445(3):231-8. DOI
19
Malcangio M, Tomlinson DR. A pharmacologic analysis of mechanical hyperalgesia in streptozotocin/diabetic rats. Pain. 1998;76(1-2):151-7. DOI
20
Levy D, Zochodne DW. Increased mRNA expression of the B1 and B2 bradykinin receptors and antinociceptive effects of their antagonists in an animal model of neuropathic pain. Pain. 2000;86(3):265-71. DOI
21
Yoon C, Wook YY, Sik NH, Ho KS, Mo CJ. Behavioral signs of ongoing pain and cold allodynia in a rat model of neuropathic pain. Pain. 1994;59(3):369-76. DOI
22
Kim KH, Tsao R, Yang R, Cui SW. Phenolic acid profiles and antioxidant activities of wheat bran extracts and the effect of hydrolysis conditions. Food Chem. 2006;95(3):466-73. DOI
23
Saija A, Tomaino A, Trombetta D, De Pasquale A, Uccella N, Barbuzzi T, Paolino D, Bonina F. In vitro and in vivo evaluation of caffeic and ferulic acids as topical photoprotective agents. Int. J. Pharm. 2000;199(1):39-47. DOI
24
Boyce-Rustay JM, Honore P, Jarvis MF. Animal models of acute and chronic inflammatory and nociceptive pain. InAnalgesia. Humana Press, Totowa, NJ. 2010;617:41-55. DOI
25
Yalcin IA, Charlet M, Freund-Mercier M, BarrotP.Differentiating Thermal Allodynia and Hyperalgesia Using Dynamic Hot and Cold Plate in Rodents. J. Pain .2009;10(7):767-73. DOI
26
Ohinishi TT, Suzuki Y, Suzuki K, Ozawa A. Comparative study of plasma membrane Mg2+ ATPase activities in normal, regenerating and malignant cells. Biochim Biophys Acta Biomembr. 1982;684(1):67-74. DOI
27
Slater TF, Sawyer BC. The stimulatory effect of carbon tetrachloride and other halogenalkane or peroxidative reaction in the rat liver functions in vitro. Biochem J. 1991; 123: 805-15. DOI
28
Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochim Biophys Acta Gen Subj. 1979;582(1):67-78. DOI
29
MISRA H. FridovichI. The role of superoxide anion in the autooxidation of epinephrine and a simple assay of SOD. J Bio Chem. 1972;247:3170-5.
30
Aebi H. Catalase in vitro. InMethods in enzymology. Academic Press. 1984;105:121-6 DOI
31
Hjertén S, Pan H. Purification and characterization of two forms of a low-affinity Ca2+ -ATPase from erythrocyte membranes. Biochim Biophys Acta Biomembr. 1983;728(2):281-8. DOI
32
Adisakwattana S, Moonsan P, Yibchok-Anun S. Insulin-releasing properties of a series of cinnamic acid derivatives in vitro and in vivo. J. Agric. Food Chem. 2008;56(17):7838-44. DOI
33
Fiske CH, Subbarow Y. The colorimetric determination of phosphorus. J. biol. Chem. 1925;66(2):375-400.
34
Busby R, Schelvis WJPM, Yu DS, Babcock GT, Marlett MA. Lipoic acid biosynthesis: LipA is an iron-sulphur protein. J Am Chem Soc. 1999;121(19):4706–7.
35
Singh U, Jialal I. Retracted: alpha-lipoic acid supplementation and diabetes. Nutr. Rev. 2008;66(11):646-57. DOI
36
Şengül M, Yildiz H, Kavaz A. The effect of cooking on total polyphenolic content and antioxidant activity of selected vegetables. Int. J. Food Prop. 2014;17(3):481-90. DOI
37
Verma V, Singh N, Singh Jaggi A. Pregabalin in neuropathic pain: evidences and possible mechanisms. Curr. Neuropharmacol. 2014 Jan 1;12(1):44-56. DOI
38
Hundehege P, Fernandez-Orth J, Römer P, Ruck T, Müntefering T, Eichler S, Cerina M, Epping L, Albrecht S, Menke AF, Birkner K. Targeting voltage-dependent calcium channels with pregabalin exerts a direct neuroprotective effect in an animal model of multiple sclerosis. Neurosignals. 2018;26(1):77-93. DOI
39
Pournaghi P, Sadrkhanlau R, Foroughi A. An investigation on body weight, blood glucose level and pituitary-gonadal axis hormones in diabetic and metformin-treated diabetic female rats. Vet Res Forum.2012;3(2):79-84.
40
Kumar N, Pruthi V. Potential applications of ferulic acid from natural sources. Biotechnol.2014;4: 86-93. DOI
41
Petya K. Improvement of insulin sensitivity in patients with type 2 diabetes mellitus after oral administration of alpha-lipoic acid. Hormones (Athens). 2014; 5(4):251-8. DOI
42
Choi R, Kim BH, Naowaboot J, Lee MY, Hyun MR, Cho EJ. Effects of ferulic acid on diabetic nephropathy in a rat model of type 2 diabetes. Exp. Mol. Med.2011;43(12):676-83. DOI
43
Howarth FC, Jacobson M, Shafiullah M, Adeghate E. Long‐term effects of streptozotocin‐induced diabetes on the electrocardiogram, physical activity and body temperature in rats. Exp. Physiol. 2005;90(6):827-35. DOI
44
Eze ED, Atsukwei D, Adams MD, Tende JA, Malgwi IS. Effects of alpha lipoic acid on blood glucose, body weight and haematological profile of streptozotocin-induced hyperglycaemia in wistar rats. Eur. J. Med. Res. 2015;3(2):25-33.
45
Kandhare AD, Raygude KS, Ghosh P, Ghule AE, Bodhankar SL. Therapeutic role of curcumin in prevention of biochemical and behavioral aberration induced by alcoholic neuropathy in laboratory animals. Neurosci. Lett. 2012;511(1):18-22. DOI
46
Bhokare KH, Upaganlawar AB. Neuroprotective effects of Lagerstroemia speciosa L. extract (Banaba Leaf Extract) in Streptozotocin Induced painful diabetic neuropathy in laboratory rats. Pharmacologia. 2016;7:9-15. DOI
47
Jorige A, Annapurna A. Neuroprotective and antioxidant role of pregabalin in streptozotocin induced neurotoxicity. Int. J. Pharm. Sci. Res.. 2016;7(11):4494.
48
Finnerup NB, Jensen TS . Clinical use of pregabalin in the management of central neuropathic pain. Neuropsychiatr. Dis. Treat.2007;3(6):885–91.
49
Rajasekaran S, Sivagnanam K, Subramanian S. Antioxidant effect of Aloe vera gel extract in streptozotocin-induced diabetes in rats. Pharmacol Rep. 2005;57(1):90-6.
50
Ueno Y, Kizaki M, Nakagiri R, Kamiya T, Sumi H, Osawa T. Dietary glutathione protects rats from diabetic nephropathy and neuropathy. The Journal of nutrition. 2002;132(5):897-900. DOI
51
Kalkan IH, Suher M. The relationship between the level of glutathione, impairment of glucose metabolism and complications of diabetes mellitus. Pak J Med Sci. 2013;29(4):938. DOI
52
Bravenboer B, Kappelle AC, Hamers FP, Van Buren T, Erkelens DW, Gispen WH. Potential use of glutathione for the prevention and treatment of diabetic neuropathy in the streptozotocin-induced diabetic rat. Diabetologia. 1992;35(9):813-7. DOI
53
Budin SB, Othman F, Louis SR, Bakar MA, Radzi M, Osman K, Das S, Mohamed J. Effect of alpha lipoic acid on oxidative stress and vascular wall of diabetic rats. Rom J Morphol Embryol. 2009;50(1):23-30.
54
Ramar M, Manikandan B, Raman T, Priyadarsini A, Palanisamy S, Velayudam M, Munusamy A, Prabhu NM, Vaseeharan B. Protective effect of ferulic acid and resveratrol against alloxan-induced diabetes in mice. Eur. J. Pharmacol. 2012;690(1-3):226-35. DOI
55