Heavy metal phytoremediation potential of CYP4502E1 expressing A. thaliana and S. grandiflora plants

Document Type: Original Article

Authors

1 Ministry of Science and Technology, Baghdad, Iraq

2 College of Biotechnology, Al-Nahrain University, Baghdad, Iraq

3 Department of Molecular Biology and Biotechnology, Atomic Energy Commission, Damascus, Syria

Abstract

Heavy metals are among the highly dangerous pollutants although various physical and chemical remediation methods are available, phytoremediation is so far one of the easiest, safest, and cheapest methods for heavy metal remediation polluted sites. In this research, heavy metal phytoremediation potentials of the previously generated CYP4502E1 expressed in A. thaliana and S. grandiflora plants were evaluated. For this purpose, both transgenic plants were grown under greenhouse-controlled conditions. Firstly, plant phytoremediation potentials were evaluated under different irrigation inputs of Mn (75, 100, and 125 ppm); Cu (75, 100, and 125 ppm); and Pb (30, 40, and 50 ppm). Additionally, plants phytoremediation potentials of Zn (41.9 ppm) and Br (51.3 ppm) removal were evaluated overtime after 5, 10, and 15 days of growth. Results showed a significant increase in Mn, Cu, and Pb plant content in both plants with increasing contaminant inputs. Pb content in pot soil where S. grandiflora remained almost constant under the increased Pb inputs, which refers to the high potentials of this plant in remediating Pb. Contaminated soil experiment also showed a significant increase in Zn and Br content in both plants over time with a significant decrease in soil contaminants contents.

Keywords


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